Molecular Biology Laboratory
Denaturing high-performance liquid chromatography (DHPLC) is a chromatographic mutation analysis technique that is based on temperature-dependent separation of DNA containing mismatched base pairs from polymerase chain reaction (PCR)-amplified DNA fragments. This is an accurate technology for detection of known and unknown mutations and single nucleotide polymorphisms (SNPs).
DGGE is a molecular fingerprinting method that separates (PCR)-generated amplicons. DNAs having different sequences, coming from complex bacterial environments, will melt at different denaturant concentrations resulting in a characteristic bands pattern. Each band theoretically represents a single bacterial population present in the community.
Real time PCR is a technique used to monitor the progress of a PCR reaction in real time. At the same time, a relatively small amount of PCR product (DNA, cDNA or RNA) can be quantified. Real time PCR assays are easy to perform, have high sensitivity and specificity.
PCR allows isolation of DNA fragments from genomic DNA by the amplification of a specific region of DNA. The PCR is the most sensitive molecular method to detect microorganisms in biological, clinical and food samples, etc.
This tool enables accurate detection and quantification of a wide range of organisms, biological molecules and samples deep characterization.
As an example the UV trans-illumination mode is used for ethidium bromide stained DNA in agarose gel/SYBR green stained DNA in agarose gel and the light trans-illumination mode for coomassie blue stained proteins in polyacrylamide gel.