Depending on customer's needs and the final applications, matrices from which a new probiotic can be isolated can also include complex and highly diversified raw materials or products.
The isolation of new bacteria can come from the most diverse matrices, just as the application of the same strain can then find its optimal expression in completely different matrices.
Tests of resistance to gastric and pancreatic juice, resistance to contact with bile salts, evaluation of strain's safety and, where required, complete sequencing of the genome of the strain.
Test on different media and matrices with the aim of highlighting the best strain performance and dominant physiological characteristics.
Batch fermentation on a laboratory scale of 5 liters and subsequent scaling up to 15 liters
Lyophilization of fermentation product and matrices under different conditions
Verification of the shelf - life of the prototype in a climatic chamber