Lactobacillus crispatus M247-derived H2O2 acts as a signal transducing molecule activating peroxisom
Sandra Voltan, Diego Martines, Marina Elli, Paola Brun, Stefano Longo, Andrea Porzionato, Raffaele DeCaro, Giorgio Palù, Giacomo C. Sturniolo, Lorenzo Morelli, Ignazio Castagliuolo (2008) Gastroenterology 135(4):1216-27.
BACKGROUND & AIMS: Accumulating evidence indicates that the peroxisome proliferator activated receptor (PPAR)-gamma is a major player in maintaining intestinal mucosa homeostasis, but whether PPAR-gamma is directly involved in probiotic-mediated effects and the molecular events involved in its activation are not known. METHODS: We investigated the role of PPAR-gamma in the immunomodulatory effects of Lactobacillus crispatus M247 on intestinal epithelial cells (IEC) and the role of probiotic-derived H(2)O(2) on PPAR-gamma activity. RESULTS: L crispatus M247 supplementation in mice significantly increased PPAR-gamma levels and transcriptional activity in the colonic mucosa. L crispatus M247 induced PPAR-gamma nuclear translocation and enhanced transcriptional activity in epithelial (CMT-93) cells, as demonstrated by the increased luciferase activity of a PPAR-gamma-responsive element, PPAR-gamma-responsive gene up-regulation, and reduced activity of an nuclear factor-kappaB-responsive element. Pharmacologic PPAR-gamma inhibition or silencing by small interfering RNA cancelled the L crispatus M247-mediated effects in CMT-93 cells. Because Lactobacillus strains producing little H(2)O(2) failed to activate PPAR-gamma, we investigated the role of L crispatus M247-derived H(2)O(2) in PPAR-gamma activation. L crispatus M247 induced a transient rise in intracellular H(2)O(2) and PPAR-gamma transcriptional activity was cancelled by antioxidant or H(2)O(2) scavenger. Toll-like receptor (TLR)-2 was not required for PPAR-gamma up-regulation mediated by L crispatus M247 in mice, although the protective effects of L crispatus M247 on dextran sodium sulfate-induced colitis were less pronounced in TLR-2(-/-) mice. CONCLUSIONS: L crispatus M247 uses H(2)O(2) as a signal transducing molecule to induce PPAR-gamma activation in IEC, directly modulating epithelial cell responsiveness to inflammatory stimuli.
Beneficial effect of auto-aggregating Lactobacillus crispatus on experimentally induced colitis
Aggregating phenotype in Lactobacillus crispatus determines intestinal colonization and TLR2 and TLR
Proteomic investigation of the aggregation phenomenon in Lactobacillus crispatus
Assessment of susceptibility towards biocides within lactic acid bacteria
Biocide susceptibility within bifidobacteria of animal and human origin
Assessment of susceptibility of moulds isolates towards biocides
Kalkanci A, Elli M, Adil Fouad A, Yesilyurt E, Jabban Khalil I. Assessment of susceptibility of moulds isolates towards biocides. J Mycol Med. 2015 Dec;25(4):280-6. doi: 10.1016/j.mycmed.2015.08.001. Epub 2015 Sep 26.